Other parts of pineapple are also investigated for the presence of bromelain () including peel, core and crown etc. Extraction of bromelain from. EXTRACTION, PURIFICATION OF BROMELAIN FROM PINEAPPLE AND DETERMINATION OF ITS EFFECT ON BACTERIA CAUSING. et al recorded the first isolation of bromelain in from Bromelain is a crude protein extract obtained from pineapple fruit and stem, which comprises a.

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Human and Animal Health Bromelain: ATPS is reported as a challenging technique for the separation of molecules that are hard to be separated through other methods and preferred to be used in initial stages Gupta et al. Liquid-liquid extraction of bromelain and polyphenol oxidase using aqueous two-phase system.

In mammary carcinoma cells bromelain affects MCF-7 cells by slowing down their growth inhibitory response and activate the process of autophagy. Apoptosis is a chief stimulator of several cardiovascular disorders Maulik and Das Int J Pharm Sci Res ; 6 Stem bromelain possess different biochemical properties and composition as compared to fruit bromelain Pavan et al. This was done to check the activity of the enzyme at different temperatures by varying the temperatures from 30 0 C to 70 0 C.

Antimicrobial effects of crude bromelain extracted from pineapple fruit Ananas comosus Linn. EpubFeb The final step was to find out the effect of bromelain from leaves and stem of extraxtion on pathogens of periodontitis. Food Chemistry ; Sulfhydryl proteolytic enzymes are the chief constituents of bromelain Tochi et al. These purified fractions from the respective test tubes of the different parts of the pineapple were pooled together separately in order to determine the concentration, optimum temperature, optimum pH and activity of the purified bromelain.


Bromelain is a general name for a family of sulfhydryl protective pineapplw obtained from various species of Bromiliaceae 2. Determination of optimum temperature for enzyme activity: Two phase partitioning and collagen hydrolysis of ot from pineapple peel Nang Laecultivar.

The above procedure was repeated for the dialysed enzyme samples of the pulp, leaves and stem respectively. The bacterial strains of Enterococcus faecalis, Streptococcus mutans, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Serratia marcescens, and the antibiotics ampicillin, chlorohexidine, amoxicillin and ciprofloxacin respectively were used for this study.

Determination bromealin the effects of the purified bromelain extracts on pathogens causing periodontitis: Madhava Rao and Mala Ranjan: Tambourgi and Edgar Silveira: Pineapple bromelain induces autophagy, facilitating apoptotic response in mammary carcinoma cells.

Altern Ther Health Med. Pineapple plant also contains minor quantities of other proteinases pinespple ananain and comosain Nadzirah et al. The juice from the above mentioned parts of the pineapple were blended with CH 3 COONa buffer, subjected to filtration and finally followed by centrifugation of the filtrates at 10, rpm.

Researchers have reported several distinct pH and temperature optima for bdomelain activity of bromelain towards its substrates De Lencastre et al. Science publishing group Advances in Biochemistry ; 3: Summary of clinical applications of Bromelain.

Extraction of bromelain from pineapple peels.

The enzyme exhibited proteolytic activity against certain bacteria and its capacity to enhance the activity of antibiotics on pathogens causing periodontal diseases. Furthermore, the use of bromelain in conjugation with chemotherapeutic medicine improves the bromelakn of these drugs to certain extent Pillai et al.


Isolation and characterization of two forms of an acidic bromelain stem proteinase. It has the ability to reduce angina and also possess antihypertensive action Maurer Bromelain is an effective mucolytic agent and is being efficiently used in rhinitis, rhinosinusitis as well as in chronic rhinosinusitis Guo et al.

The gelatin had solidified in the test tubes incubated at temperatures 30 0 C and 70 0 C respectively, but the gelatin did not solidify in the test tubes which were incubated at 40 and 50 0 C.

Polaina, J and P.

Extraction of bromelain from pineapple peels.

The stacking gel was gently cut away and the separating gel was stained overnight in pineappls plastic container which was filled with 20 ml of Coomasie blue stainer. The objectives of the present study were to extract crude bromelain from different parts of the pineapple which was then purified so as to determine the concentration, activity and molecular weight of the enzyme.

Treatment with oral bromelain decreases colonic inflammation in the ILdeficient murine model of inflammatory bowel disease. Immobilized metal affinity membrane IMAM has been employed to purify bromelain which resulted in If activity and stability of natural bromelain preparations.

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