Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.

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The development, optimization and evaluation of a polymerase chain reaction PCR assay are presented for the diagnosis of pseudorabies infection.

Each one of the nine tissue field samples from pigs diagnosed as PRV infected based on clinical signs and laboratory methods yielded the corresponding PRV amplified product when analyzed. The analytical sensitivity of the test was estimated to be 1. The polymerase chain reaction PCR is a rapid tool that can be used no only to detect acutely PRV infected pigs but it is the recommended test for detect PRV latent infection.

A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided. Table 1 Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. The polymerase chain reaction PCR can be used to identify PRV genomes in secretions or organ samples and although some PCR assays for PRV detection with different sensitivities have been reported 37915 there is no standard procedure recommended so far 2.

Diagnostic methods for detection of Classical swine fever virus—Status quo and new developments. A rapid and accurate diagnosis of PRV infection is important for the initiation of appropriate control strategies.

Doença de Aujeszky – Wikipédia, a enciclopédia livre

PCR experiments were performed on serial ten-fold dilutions of a wuinos suspension of PRV isolate V with a titer of 10 6. The virus primarily replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected.


The PCR assay described here provides a rapid, highly sensitive, and cost-effective laboratory diagnosis for pseudorabies infections. This paper describes the development, optimization and performance assessment of a rapid and highly sensitive PCR test for detection of pseudorabies virus.

World Organization for Animal Health. Induction and inhibition of apoptosis by pseudorabies virus in the trigeminal ganglion voena acute infection of swine. Oligonucleotide primers and restriction endonuclease selection PRV specific primers were designed using the Oligo 6. The isolation and characterization of a strain of infectious bovine rhinotracheitis virus from stillbirth in swine. Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs.

Under typical conditions of intensive swine aujeskzy, several clinically similar viral diseases can occur which require laboratory differential diagnosis.

Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier. Primers sequences, genome positions and the size of PCR products are shown in Table 1. Traditionally, PRV detection is based on direct virus isolation followed by confirmation dena immunofluorescence, immunoperoxidase or neutralization tests with specific antiserum 2.

This region was highly conserved for all reported genomes as shown by aligning of these sequences. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.

Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with en on the respiratory tract. The viral agent following a primary replication can establish latent infection ddoena develops a latency-reactivation infection which allows its perpetuation in pig populations 1012 However, this method is time-consuming and false negative results may occur in submissions from latently infected animals Specificity of the PRV amplicons was furthermore confirmed by Suinod I restriction endonuclease analysis which generated the two expected fragments of and bp in length Fig.

Iowa State University Press; The assay proved to be very sensitive due to as little as 1. Seven tissue samples from clinically healthy animals were negative for PCR amplification data not shown.

This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs The Sma I restriction endonuclease site was used for additional specificity confirmation of the amplification products.

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The effect of temperature and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction.

Moreover, the viral genomes of a related herpesvirus and other DNA genome porcine viruses as follow: The possibility to perform annealing and elongation in one single step of the thermal profile contributed to the specificity and the efficiency of the assay and allowed the use of a very fast PCR program. The virus principally affects pigs, which are considered to be the natural host for PRV and the reservoir of the virus in nature, but also infects a broad range of wild and non-porcine mammals with the important exception of higher-order primates 8.

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The assay specificity was demonstrated by the absence of amplifications in all heterologous viruses evaluated and in tissue samples derived from seven healthy pigs. This assay was based on the amplification of a highly conserved viral gD gene fragment.

Since the rapid detection of infected animals would reduce the potential transmission of the se to uninfected herds avoiding the spread of the diseases In general, PRV infections must be considered in the differential diagnosis of respiratory, reproductive and nervous disorders.

Finally, nucleic acids from tissue homogenates samples derived from seven healthy pigs, and a non infected PK cell line were also tested showing no positive products data not shown.

Doença de Aujeszky

Replication in the respiratory tract, central nervous system and reproductive organs is responsible for pathological changes causing different disorders National Center for Biotechnology InformationU. Agarose gel electrophoresis was used to detect PCR products.

The annealing temperature and number of cycles were determined experimentally.

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