Adhatoda vasica belonging to Acanthaceae family, commonly known as Adosa, Adhatoda vasica Flowers, Antibacterial activity, Antifungal activity, Diffusion. Abstract. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Antibacterial activity of leaf extract of Adhatoda vasica was studied against Bacillus subtilis, Antibacterial activity was determined by disc diffusion method.

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Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Nees Acanthaceaeknown commonly as Malabar nut tree, is a shrub growing throughout the Indian peninsula. Journal of Obstetrics and Gynaecology of India. Vasicine acetate showed moderate antibacterial activity antiabcterial to Vasicine. Escherichia coli was highly resistant to all the plant extracts at all concentrations.

Extraction Shade dried and coarsely powdered leaves of A.

Antibacterial Activity of Leaf Extracts of Adhatoda vasica – MAHE Digital Repository

Vasicine acetate showed antimycobacterial activity [ 15 ]. The MIC for bacteria was determined as the lowest concentration of the compound anntibacterial the visual growth of the test cultures on the agar plate. Classical Uses of Medicinal Plants. The cytotoxicity was determined according to the method of Balachandran et al.

Instruments All melting point values are uncorrected and taken by open capillary method on a heating block Instrument.

The following Gram negative, Gram positive bacteria, clinical isolates, and fungi were used for the experiment: The antifungal agent Ketoconazole for fungi and antibacterial agent Streptomycin for xntibacterial were included in the assays as positive controls. Adhatoda vasica, Bauhenia variegate, Bombax ceiba, Carrisa opaca, Caryopteris grata, Debregeasia salicifolia, Lantana camara, Melia azedarach, Phyllanthus emblica, Pinus roxburghii and Olea ferruginea collected from lower Himalayas against two Gram positive Staphylococcus aureus, Micrococcus luteus and two Gram negative Escherichia coli, Pseudomonas aureginosa bacterial strains.


In vitro antibacterial activity of selected medicinal plants from lower Himalayas.

The spot turned orange red on anfibacterial with Dragendorff’s reagent. Antifeedant and toxic activity of Adhatoda vasica leaf extract against Spodoptera littoralis Lep. Minimum Inhibitory Concentration MIC Minimum inhibitory concentration studies of the compounds were performed according to the standard reference methods for bacteria [ 17 ] and filamentous fungi [ 18 ]. The emergence of drug-resistant pathogens and the increase in diseases affecting the immune system have greatly intensified the need to investigate new bioactive metabolites for potential pharmaceutical and industrial applications [ 12 ].

Minimum inhibitory concentration studies of the compounds were performed according to the standard reference methods for bacteria [ 17 ] and filamentous fungi [ 18 ]. Glossary of Indian Medicinal Plants. The plant is used in the indigenous system of medicine in India and is a well-known expectorant in both Ayurvedic and Unani Systems of Medicine [ 45 ].

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted axhatoda, distribution, and reproduction in any medium, provided the original work is properly cited.

It was studied for antimicrobial, antioxidant, and anticancer activities. Indian Journal of Natural Products and Resources.

Antimycobacterial activity of two natural alkaloids, vasicine acetate and 2-acetyl benzylamine, isolated from Indian shrub Adhatoda vasica Ness. Greater antibacterial activity against Pseudomonas aeruginosa was only exhibited by Phyllanthus emblica, Pinus roxburghii, Debregeasia salicifolia and Lantana camara.


The mobile phase was filtered through a 0.

The worldwide threat of antimicrobial resistance. The physical and spectroscopic data were compared with those reported in the literature [ 15 ].

National Center for Biotechnology InformationU. Preliminary phytochemical and antibacterial screening of crude extract of the leaf of Adhatoda vasica. Support Center Support Center. Results and Discussion The ethanol extract of A.

Other constituents include vitamin C, saponins, flavonoids as well as steroids, and fatty acids [ 13 ]. Antibacterial activities against Staphylococcus aureus and Micrococcus luteus were observed formethanolic extracts of all the above mentioned plants.

Indian Journal of Medical Research. The physical and spectroscopic data were compared with those reported in the literature [ 2122 ]. Vasicine acetate showed minimum inhibitory concentration values against bacteria; they were M. Structure of the compounds: International Journal of Cancer. Further elution of the column with chloroform: Medicinal plants are important sources of practical drugs for people throughout the year.

HPLC analysis was carried out on a Waters Axhatoda separations module with photodiode array detector Waters, Vasicine acetate was obtained by acetylation of Vasicine. Nature acts as a prominent reservoir for new and novel therapeutics. The combined chloroform extract was washed with water, dipped, and dried over anhydrous sodium sulphate. A human adenocarcinoma cancer cell line was qntibacterial from National Institute of Cell Sciences, Pune.

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